Latest update
I haven't posted an update for several months so I wanted to let you know that we are still working on this project and are trying several different methods to obtain Brca1 mutant pups. First, the ...
Failure to breed
You may remember from my last update that we had managed to obtain one Brca1 heterozygous mutant pup, which we planned to breed to get enough mice for the PARP inhibitor experiment. Unfortunately I...
We have a Brca1 mutant heterozygote!
Dr. Cowley gave me another update on the status of the breeding of the Brca1 mutant mice today: "I just wanted to let you know that 1 of the 4 surviving pups is a Brca1 heterozygote female. We hav...
The pups are born!
Dr. Cowley gave me an update on the status of the breeding today: "We had 5 pups born Tuesday from the second round of Brca1 transfers. One pup died, but the other 4 are doing well. Hopefully we w...
Pups update
Last month I wrote about the problem we had obtaining viable Brca1 mutant pups (https://www.microryza.com/projects/can-we-prevent-the-transmission-of-brca-mutations/updates/262/). Unfortunately o...
Pups
Several weeks ago we obtained frozen embryos from the NCI to breed our Brca1 null mutated mice for the study: http://mouse.ncifcrf.gov/available_details.asp?ID=01XC4Dr. Cowley gave me an update on ...
Revised fundraising goal!
I am revising my fundraising goal down from $15,000 to $10,000 which means... I MADE IT! So this project will definitely go ahead! I was very fortunate to be able to obtain the Brca1 mutant mice fr...
Twitter ideas
It is always useful to get feedback about your experimental design so it was really great to get an additional experiment suggested by @boehninglab via twitter for my research project (I have attac...
Detailed experimental design
Detailed Experimental DesignPower calculation:55 pups will be bred and genotyped per group to give an 80% probability of showing a statistically significant difference (using p < 0.05) of detect...